Sox10-GFP/DTA transgenic mice

To generate the Sox10-lox-eGFPpolyA-lox-DTA transgene, we used a 120Kb NotI fragment from a Sox10 genomic PAC isolated from library RCPI-21 from the UK HGMP Resource Centre. The library contains genomic DNA from a female mouse strain 129S6/SvEvTac (Osoegawa 2000). The 120kb region included all Sox10 coding exons, as well as ~60kb upstream and ~50kb downstream.

We replaced the entire Sox10 open reading frame with a floxed eGFP-SV40polyA4 cassette, followed by an attenuated G383A version of DTA (from Ian Maxwell, University of Colorado, Denver). In the developing and adult mouse, eGFP expression can be detected in Sox10-expressing cells, including oligodendrocyte-lineage cells in the CNS (Fig.1). In the presence of Cre recombinase, the eGFP-polyA4 cassette is removed, activating the DTA cassette and killing the expressing cells. Founder transgenics were generated by pronuclear injection and one line selected for propagation (“Sox10-GFP/DTA”). Generation of the mice is described in Kessaris et al. 2006 and Iannarelli 2017 and a further example of their use in Xing et al. 2023.

 _{Fig. 1. (a) intron/exon structure of the Sox10 locus (top). The genomic region spanning exons III – V was replaced with the cassette lox-eGFP-polyA4-lox-DTA-frt-Cmr-frt by homologous recombination in bacteria. The Cmr cassette was then removed by transient activation of Flp recombinase in bacteria, producing a latent DTA transgene that expresses GFP under Sox10 transcriptional control. In the presence of Cre recombinase the GFP-polyA cassette is removed, thus activating the DTA cassette and killing the expressing cells. (b-e) Coronal section through the telencephalon of a Sox10-DTA transgenic mouse at P3 and high-mag images of the cortex from the same section. All Sox10+ lineage cells in the Sox10-GFP/DTA mouse co express GFP, demonstrating the veracity of transgene expression. (f-k) The DTA transgene was activated by crossing Sox10- GFP/DTA to an Olig2-Cre mouse, excising the GFP cassette in all Olig2-expressing precursors and their descendants, hence activating expression of DTA and highly specific killing of all Sox10+ OL lineage cells, as demonstrated by the loss of all (Sox10+, GFP+) cells in the double-transgenics (compare f-h with i-k).}

Placing an order on XIP

To license this product, please select the appropriate licence option on the right-hand side of this page. Terms can be previewed from the "Preview terms" link.

MTAs require agreement between all the parties involved in supplying and receiving a product. This cannot happen instantaneously but is a controlled process, managed through XIP and should not take longer no than 10 business days in ordinary circumstances.

To place an order, please locate the Sign-in or Register options on the top right side of this page. You can either sign in to your existing account or register for a new now.

For additional guidance on how to create an account and place an order, refer to the FAQs. Please note that your account should be created using your academic/ institutional e-mail address.