Spontaneously immortalized human Müller glia cell line
Technology No. 88-012

Cells grow indefinitely under adherent conditions in the presence or absence of extracellular matrix proteins. Optimal medium for cell growth: DMEM medium containing high glucose and stable glutamine, supplemented with 10% foetal bovine serum. 

Under normal culture conditions, MIO-M1 express markers of mature Müller cells, including cellular retinaldehyde binding protein [CRALBP], glutamine synthetase, vimentin and epidermal growth factor receptor-[EGF-R].

The cells are NOT intended for Stem cell Research.


Size: Cells are shipped growing in 25cm2 flasks.

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The following statemeFull acknowledgement of the source of the MIO-M1 cells must be given in all relevant publications and presentations of research results.

The following statement must be included in all publications and the accompanying reference must be cited:

'The human Müller cell line Moorfields/Institute of Ophthalmology- Müller 1 (MIO-M1) was obtained from the UCL Institute of Ophthalmology, London, UK.' 

The reference is:

'Limb GA, Salt TE, Munro PMG, Moss SE and Khaw PT. (2002) In vitro characterization of a spontaneously immortalized human Müller cell line (MIO-M1). Investigative Ophthalmology and Visual Science, 43: 864-869'

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  • swap_vertical_circlelibrary_booksReferences (12)
    1. KHAW, MOSS, MUNRO, SALT, LIMB (2002), In vitro characterization of a spontaneously immortalized human Muller cell line (MIO-M1), Invest Ophthalmol.Vis.Sci. , 43(3), 864-869
    2. LIMB, KHAW, LUTHERT, REH, KEEGAN, BRATIA, SINGHAI, LAWRENCE , MIO-M1 cells and similar muller glial cell lines derived from adult human retina exhibit neural stem cell characteristics, Stem Cells , 25(8), 2033-2043
    3. CURTIS, STITT, UCHIDA, LIMB, MEDINA, ZONG, SYONG (2010), Evidence supporting a role for N-(3-formyl-3,4-dehydropiperidino)lysine accumulation in Muller glia dysfunction and death in diabetic retinopathy, Mol Vis,, 16, 2524-38
    4. STITT, CURTIS, LIMB, YONG, MADDEN, WARD, ZONG (2010), Hyperglycaemia-induced pro-inflammatory responses by retinal Muller glia are regulated by the receptor for advanced glycation end-products (RAGE), Diabetologia,, 53, 2656-66.
    5. MARTIN, LIMB, BULL (2008), Human Muller stem cell (MIO-M1) transplantation in a rat model of glaucoma: survival, differentiation, and integration, Invest Ophthalmol Vis Sci,, 49, 3449-56
    6. BRINGMANN, WIEDEMANN, KOHEN, LIMB, JAHN, HOLLBORN (2004a), Characterization of the basic fibroblast growth factor-evoked proliferation of the human Muller cell line, MIO-M1, Graefes Arch Clin Exp Ophthalmol,, 242, 414-22
    7. BRINGMANN, WIEDEMANN, WOLF, KOHEN, REICHENBACH, LIMB, SCHNURRBUSCH, BIGL, TENCKHOFF, YAFAI, IANDIEV, KRAUSSE, HOLLBORN (2004b), Glial cell expression of hepatocyte growth factor in vitreoretinal proliferative disease, Lab Invest,, 84, 963-72.
    8. BRINGMANN, KOHEN, WIEDEMANN, WOLF, REICHENBACH, LIMB, SCHNURRBUSCH, BIEDERMANN, IANDIEV, JAHN, TENCKHOFF, HOLLBORN (2005), Changes in retinal gene expression in proliferative vitreoretinopathy: glial cell expression of HB-EGF, Mol Vis,, 11, 397-413
    9. KOHEN, BRINGMANN, LIMB, WIEDEMANN, REICHENBACH, WAGNER, WURM, DUKIC-STEFANOVIC, RILLICH, ULBRICHT, HOLLBORN (2011), The human Muller cell line MIO-M1 expresses opsins, Mol Vis,, 17, 2738-50
    10. ZHANG, SONG, ZHOU, SUN, YANG, PENG, XIE, LIMB, HU, XIONG, YE, LIU (2014), IL-1beta Upregulates IL-8 Production in Human Muller Cells Through Activation of the p38 MAPK and ERK1/2 Signaling Pathways, Inflammation, ,
    11. KENNEY, KUPPERMANN, LIMB, LUCZY-BACHMAN, GUPTA, MANSOOR (2010), Protective effects of memantine and epicatechin on catechol-induced toxicity on Muller cells in vitro, Toxicology,, 271, 107-14
    12. KENNEY, KUPPERMANN, LIMB, CHWA, FRANCO, PHAM, RAMIREZ (2013), Hydroquinone induces oxidative and mitochondrial damage to human retinal Muller cells (MIO-M1), Neurotoxicology,, 39, 102-8
  • swap_vertical_circlecloud_downloadSupporting documents (0)
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Material Transfer Agreement - MIO-M1
For non-commercial research by non-profit organisations. Continue to checkout to see shipping prices.

Term: 24 months

Price per unit:
£800.00 excl. VAT

Material Transfer Agreement - MIO-M1 (No Shipping of Samples Required)
For non-commercial research by non-profit organisations. No shipping cost.

Term: 24 months

Price per unit:
£800.00 excl. VAT

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