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GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.


Greenwood et al., (1996) SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology 71: 51-63. PMID: 8982103.

Regina et al., (1999) Dexamethasone regulation of P-glycoprotein activity in an immortalized rat brain endothelial cell line, GPNT. Journal of Neurochemistry 73: 1954-1963.PMID:10537053







An immortalised Lewis rat brain vascular endothelial cell line

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